ukncc
  Testing: scope of testing carried out and standards followed  
 

Several of the UKNCC collections carry out testing of materials and of the efficacy of biocides. The following summarises the testing and in many cases the standards used. However, if the test or procedure is not listed do not hesitate to contact any of the collections that hold the organisms you wish to use or carry out the type of tests you require.

Microbial resistance testing

CABI
NCWRF
Testing of materials and equipment to NAMAS standards

Microbial biocide testing

CABI
Testing to customer specification or against a set of standard test strains (usually around 50 species). Testing carried out to NAMAS standards.

NCIMB
Efficacy of microbial biocides. E.g., Testing of biocidal chemicals against planktonic and sessile populations of sulphate reducing bacteria (SRB) in specialist laboratory apparatus. Allows selection of appropriate formulations under simulated field conditions and the optimization of treatment protocols. Other bacteria systems can be developed as contract research. Testing carried out to ISO 9000 standards

CCAP
The following strains are routinely employed by industry to test new biocides, paint additives etc.

  • Trentepohlia aurea CCAP 483/1
  • Trentepohlia odorata CCAP 483/4
  • Stichococcus bacillaris CCAP 379/1A
  • Nostoc commune CCAP 1453/29
  • Nostoc commune CCAP 1453/24
  • Nostoc muscorum CCAP 1453/8
  • Nostoc muscorum CCAP 1453/12
  • Nostoc muscorum CCAP1453/20
  • Oscillatoria tenuis CCAP 1459/4
  • Anacystis montana CCAP 1430/1
  • Pleurococcus sp. CCAP 464/1
  • Chlorella emersonii v. globosa CCAP211/8C
  • Chlorella emersonii CCAP 211/8G
  • Scenedesmus fusca CCAP 211/23
  • Stigeoclonium tenue CCAP 477/11A
  • Gloeocapsa sp. CCAP 1430/2
  • Other algae are routinely used as bioassay organisms including: Ochromonas danica CCAP 933/2B (Thiamin & biotin bioassay) and Euglena gracilis CCAP 1224/5Z (vitamin B12 bioassay)

    Key references: OECD, 1984, OECD guidelines for testing chemicals Section 2 - Effects on biotic systems, No. 201, "Alga, growth inhibition test". Paris: Organization for Economic Development. EEC, 1987, EC Directive 87/302/EEC

    CABI
    Culture extract supply for screening

    Ecotoxicity testing

    CCAP
    All new chemical products that are likely to be released into the environment must undergo a series of standard internationally agreed algal growth inhibition toxicity tests (OECD, 1984, EEC, 1987). Microalgae utilised in these tests are housed in the major culture collections and include the following strains:

    • Chlorella vulgaris CCAP 211/11B = SAG 211-11b = UTEX 259
    • Chlorella vulgaris CCAP 211/12 = SAG 211-12 = UTEX 30 = ATCC 16487
    • Selenastrum capricornutum CCAP 284/4 = UTEX 1648 = ATCC 226622
    • Scenedesmus subspicatus CCAP 276/20
    • Scenedesmus subspicatus CCAP 276/22 = SAG 86.81 = UTEX 2594
    • Phaeodactylum tricornutum CCAP 1052/1A
    • Skeletonema costatum CCAP 1077/3
    • Skeletonema costatum CCAP 1077/5 = CCMP 1332